Corning® Collagen I, rat tail, 100mg, is used either as a thin layer on tissue-culture surfaces to enhance cell attachment and proliferation, or as a gel to promote expression of cell-specific morphology and function.

 

Source

Rat Tail Tendon

 

Size

100mg

 

Quality

Purity = 90% by SDS-PAGE

Tested for: ability to promote attachment and spreading of HT-1080 human fibrosarcoma cells

Forms firm gel up to a dilution of 1:10; further dilution will decrease rigidity of gel

Tested and found negative for bacteria, fungi, and mycoplasma

 

Formulation

Liquid in 0.02 N acetic acid, concentration range 3-4mg/ mL

 

Preparation and Storage

Stable for at least three months at 2° to 8°C. Do not freeze.

 

Handling

Effective as a gel or thin coating at a recommended concentration of 5-10µg/cm² of growth surface depending on cell type