商品详情：Corning® Matrigel® Growth Factor Reduced (GFR) Basement Membrane Matrix, 5 mL, is suited for applications requiring a highly defined basement membrane preparation. Available in standard GFR, High Concentration (HC), and Phenol Red-free formats. Corning Matrigel matrix is a solubilized basement membrane preparation extracted from the Engelbreth-Holm-Swarm (EHS) mouse sarcoma, a tumor rich in extracellular matrix proteins, including Laminin (a major component), Collagen IV, heparan sulfate proteoglycans, entactin/nidogen, and a number of growth factors.

Quality

-Mouse colonies routinely screened for pathogens via Mouse Antibody Production (MAP) testing

-Extensive PCR testing is performed to screen for a number of pathogensTested and found -negative for bacteria, fungi, and mycoplasma

-Protein concentrations determined by Lowry method

-Endotoxin units measured by Limulus Amoebocyte Lysate assay

-Matrigel Matrix gel stability is tested for a period of 14 days at 37°C

-Biological activity is determined for each lot using a neurite outgrowth assay.

Source

EHS mouse tumor

Application

-Cell Growth and Differentiation

-Metabolism/Toxicology Studies

-Invasion Assays

-in vitro and in vivo Angiogenesis Assays

-in vivo Angiogenesis Studies and Augmentation of Tumors in Immunosuppressed Mice

Preparation and Storage

Store at -20°C. Avoid multiple freeze-thaws.