产品编号: | MF3012 | 产品名称: | 热销:Percoll细胞分离液 | Cas No.: | |
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纯度/规格: | 17-0891-09分装 | 品牌: | 麦格 | 包装: | 100mL |
此产品是进口GE品牌17-0891-09的分装款
产品优点:
Percoll渗透性低、不穿透细胞、密度高、无毒害,是目前较理想的介质。Percoll密 度梯度离心已被多次成功地用于动物细胞及其细胞器的分离,纯化了包括人血液细胞、骨髓细胞、红血球细胞、白血球细胞、淋巴细胞、肝细胞等在内的十余种动物细胞。
注意事项:
1、Percoll细胞分 离液在储存及孵育过程中避免将试剂保留在强光中。
2、试剂瓶盖需盖紧,防止蒸发和污染。
3、本产品无毒性,无菌且可以高温高压灭菌,但稀释后不可以灭菌。
品牌厂家产品介绍如下:
Percoll PLUS/Percoll
CELL PREPARATION
Percoll™ PLUS is a silica-based colloidal medium for cell separation by density gradient centrifugation. The silica particles of the medium are covalently coated with silane, providing product stability and long shelf life. The silane coating also provides low osmolality and toxicity, as well as low viscosity. Percoll PLUS has low levels of endotoxins, making it well-suited for cell separation
in clinical research applications. Cell separation with the medium is performed under gentle conditions, facilitating the isolation
of a variety of cells, subcellular particles, and viruses where preservation of viability and morphological integrity is important. The low toxicity of Percoll PLUS ensures that removal of the medium from separated cellular particles is not usually necessary.
After adjustment, Percoll PLUS forms iso-osmotic gradients within the density range of 1.0 to 1.3 g/ml. This density range is especially useful since most cells, subcellular particles, and
viruses have a buoyant density of 1.0 to 1.2 g/ml in Percoll PLUS.
Percoll PLUS offers:
Low endotoxin levels (max. 2 EU/ml)
Absence of toxicity for cells and very low chemical reactivity
Low osmolality: Percoll PLUS can easily be adjusted with physiological saline, other balanced salt solutions, or cell culture media, to give gradients that are iso-osmotic throughout
Low viscosity resulting in rapid formation of gradients and particle separation at low centrifugal forces
Specifications for Percoll PLUS are shown in Table 1.
Percoll PLUS gradients
Percoll PLUS can be used in density centrifugation applications for the isolation and purification of cells, subcellular particles, and viruses down to ~70S. The medium offers both high resolution and biological compatibility. The low intrinsic osmolality and viscosity are particularly useful when isolating organelles where maintenance of membrane integrity is important.
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1.本产品仅供科研使用。请勿用于医药、临床诊断或治疗,食品及化妆品等用途。请勿存放于普通住宅区。
2.为了您的安全和健康,请穿好实验服并佩戴一次性手套和口罩操作。
3.实验结果可由多种因素影响,相关处理只限于产品本身,不涉及其他赔偿。
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